Advanced Glycated End Products Alter Neutrophil Effect on Regulation of CD 4 + T Cell Differentiation Through Induction of Myeloperoxidase and Neutrophil Elastase Activities

In this study, we investigated the effect of neutrophils stimulated with advanced glycated end products (AGEs), the marker of diabetes, on CD4+ T cell differentiation and its underlying mechanism. Our data showed that the cultural medium of healthy adult neutrophils treated with AGEs increased expressions of both Th1 (IFN- γ) and Th17 (IL-17) phenotypes and the transcription factors of Th1 (Tbet) and Th17 (ROR γt) in naive CD4+T cells and CD4+CD25+FoxP3+ (Treg) T cellsin vitro. Next, we found that AGEs induced the generations of myeloperoxidase (MPO) and neutrophil elastase (NE) in neutrophils; inhibition of MPO or NE attenuated the effect of AGE-stimulated neutrophils on CD4+ T cell bias. Furthermore, receptor for AGEs (RAGE) inhibitor interrupted AGE-induced MPO and NE expressions, but...

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