Efficient CRISPR/Cas9-Mediated Mutagenesis in Primary Murine T Lymphocytes.

This article outlines a protocol for CRISPR/Cas9-mediated mutagenesis in primary T lymphocytes from Cas9 transgenic mice using retroviral delivery of guide RNAs. © 2018 by John Wiley & Sons, Inc. PMID: 30312021 [PubMed - as supplied by publisher] (Source: Current Protocols in Immunology)

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