Clostridioides difficile in the environment, food, animals and humans in southern Italy: Occurrence and genetic relatedness

Publication date: Available online 11 September 2018Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Vincenza Romano, Vincenzo Pasquale, Ludovic Lemee, Imane El Meouche, Martine Pestel-Caron, Federico Capuano, Pasqualina Buono, Stefano DumontetAbstractOne hundred and thirty-eight C. difficile isolates from different sources (66 from the environment, 36 from animals, 9 from food and 27 from humans) were ribotyped by capillary electrophoresis PCR ribotyping (CE-PCR). A multilocus variable tandem repeat analysis (MLVA) was carried out on a sample subset.The most frequently isolated PCR ribotypes were 126 (15.9%), 078 (14.5%), 011/018 (11.6%), 014/020/077 (10.1%), and 010 (2.8%). In particular, strains of PCR ribotype 011/018 were isolated from human, raw milk and...

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