Differential antiviral activity of European sea bass interferon-stimulated 15 protein (ISG15) against RGNNV and SJNNV betanodaviruses

Publication date: Available online 7 September 2018Source: Fish & Shellfish ImmunologyAuthor(s): Patricia Moreno, Daniel Alvarez-Torres, Esther Garcia-Rosado, Juan J. Borrego, M. Carmen AlonsoAbstractISG15 is an antiviral protein acting intracellularly, by conjugation to viral or cellular proteins, or extracellularly, as cytokine. In this work, an in vitro system, consisting of E-11 cells over-expressing European sea bass ISG15 (Dl_ISG15_E11 cells), has been developed to evaluate the European sea bass ISG15 protein activity against RGNNV and SJNNV isolates. Regarding RGNNV, RNA2 copy number and viral titres were similar in E-11 and Dl_ISG15_E11 cells, and the cellular survival analyses demonstrated that Dl_ISG15_E11 cells were not protected against this virus. In contrast, ISG15 co...

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