Nitrogen topdressing timing modifies the gluten quality and grain hardness related protein levels as revealed by iTRAQ

Publication date: Available online 13 October 2018Source: Food ChemistryAuthor(s): Yingxin Zhong, Weiling Wang, Xin Huang, Mingming Liu, Kim Henrik Hebelstrup, Donglei Yang, Jian Cai, Xiao Wang, Qin Zhou, Weixing Cao, Tingbo Dai, Dong JiangAbstractNitrogen fertilization regimes significantly affect both grain quality and yield. Wheat plants were subjected to different application timing of topdressed nitrogen at the emergence of the top fifth (TL5), top third (TL3) and top first leaf (TL1), respectively. The iTRAQ (isobaric tag for relative and absolute quantitation) technology was adopted to obtain the complete proteome of wheat flour and to identify the differentially expressed proteins (DEPs) as regulated by nitrogen topdressing timing. Collectively, 591 proteins into 17 functional cate...

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