FRET-based dual channel fluorescent probe for detecting endogenous/exogenous H2O2/H2S formation through multicolor images

Publication date: Available online 26 December 2018Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Nithya Velusamy, Natesan Thirumalaivasan, Kondapa Naidu Bobba, Arup Podder, Shu-Pao Wu, Sankarprasad BhuniyaAbstractWe have developed a FRET-based fluorescent probe (PHS1) as a combination of two different fluorophores (coumarin and naphthalimide); which can detect both exogenous and endogenous H2S and H2O2 in live cells through multicolor images. The precise overlap between UV-absorption of naphthalimide and the emission band of coumarin in probe PHS1 allows the acquisition of the self-calibrated information of dual analytes through FRET-based imaging. The UV–Vis absorption (λabs 390 nm) and fluorescence emission (λem 460 nm) of probe PHS1 in the presence of H...

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