High glucose affected respiratory burst activity of peripheral leukocyte via G6PD and NOX inhibition in Megalobrama amblycephala

Publication date: Available online 11 September 2018Source: Fish & Shellfish ImmunologyAuthor(s): Xin Huang, Ling-Hong Miao, Yan Lin, Wen-Jing Pan, Ming-Chun Ren, Xian-Ping Ge, Bo Liu, Qun-Lan ZhouAbstractHigh glucose levels are known to impair growth and immune function in fish. Here we investigated the role of glucose-6-phosphate dehydrogenase (G6PD) and NADPH oxidase (NOX) in high glucose-associated impairment of leukocyte respiratory burst activity in Megalobrama amblycephala. We cultured peripheral leukocytes isolated from M. amblycephala with media containing no glucose (non-glucose group), 11.1 mmol/L d-glucose (physiologic glucose group), 22.2 mmol/L d-glucose (high-glucose group), or 11.1 mmol/L d-glucose + 100 μmol/L dehydroepiandrosterone (DHEA) (DHEA-treated group). A...

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