Publication date: Available online 10 September 2018Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): G.M. Proshkina, E.I. Shramova, O.N. Shilova, A.V. Ryabova, S.M. DeyevAbstractPhotodynamic therapy (PDT) is a clinical, minimally invasive method for destroying cancer cells in the presence of a photosensitizer, oxygen, and a light source. The main obstacle for the PDT treatment of deep tumors is a strong reduction of the excitation light intensity as a result of its refraction, reflection, and absorption by biological tissues. Internal light sources based on bioluminescence resonance energy transfer can be a solution of this problem. Here we show that luciferase NanoLuc being expressed as a fusion protein with phototoxic flavoprotein miniSOG in cancer cells in the pre...
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