Design, biological activity and signaling pathway of bovine consensus omega interferon expressed in Pichia pastoris

Publication date: February 2019Source: Molecular Immunology, Volume 106Author(s): Mingchun Gao, Yongli Guo, Xiuxin Luo, Jiwen Du, Yujiao Wang, Chong Cao, Junwei Wang, Wenyu HanAbstractThe bovine IFN-ω (BoIFN-ω) multigene family is located on chromosome 8, which has 14 potential functional genes and 10 pseudogenes. After aligning 14 BoIFN-ω subtypes and assigning the most frequently occurring amino acids in each position, one artificial consensus BoIFN-ω (CoBoIFN-ω) gene was designed, optimized and synthesized. Then, CoBoIFN-ω was expressed in Pichia pastoris, which was demonstrated to have 3.94-fold and 14.3-fold higher antiviral activity against VSV on MDBK cells than that of BoIFN-ω24 and BoIFN-ω3, respectively. Besides this, CoBoIFN-ω was confirmed to have antiviral activity ag...

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