Differential surface contact killing of pristine and low EPS Pseudomonas aeruginosa with Aloe vera capped hematite (α-Fe2O3) nanoparticles

Publication date: November 2018Source: Journal of Photochemistry and Photobiology B: Biology, Volume 188Author(s): Khursheed Ali, Bilal Ahmed, Mohammad Saghir Khan, Javed MusarratAbstractBiogenic hematite (α-Fe2O3) nanoparticles (NPs) of average size <10 nm were synthesized using green approach with Aloe vera extract (ALE). The aim of the study was to assess the protective effect of extracellular polymeric substances (EPS) against antibacterial and antibiofilm activities of ALE-α-Fe2O3NPs in normal EPS producers (pristine) and experimentally modified (low-EPS) Pseudomonas aeruginosa (P. aeruginosa) cells and the mechanism of cell killing. Formation of ALE-α-Fe2O3NPs has been validated by X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy ...

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