Next-generation sequencing reveals new insights about gene usage and CDR-H3 composition in the horse antibody repertoire

Publication date: January 2019Source: Molecular Immunology, Volume 105Author(s): Taciana Conceição Manso, Michele Groenner-Penna, João Carlos Minozzo, Bruno Cesar Antunes, Gregory C. Ippolito, Franck Molina, Liza F. FelicoriAbstractHorse serum antibodies have been used for greater than a century for the treatment and prophylaxis of infectious diseases and envenomations. Little is known, however, about the immunogenetic diversity that produces horse serum antibodies. Here, we employed next-generation sequencing for a first-in-kind comprehensive analysis of the equine B-cell repertoire. Nearly 45,000 and 30,000 clonotypes were obtained for the heavy-chain (IGH) and lambda light-chain (IGL) loci, respectively. We observed skewed use of the common subgroups IGHV2 (92.49%) and IGLV8 (82.50%)...

from #Head and Neck by Sfakianakis via simeraentaxei on Inoreader https://ift.tt/2QBooLm