An MBT domain containing anti-lipopolysaccharide factor (PtALF8) from Portunus trituberculatus is involved in immune response to bacterial challenge

Publication date: Available online 6 October 2018Source: Fish & Shellfish ImmunologyAuthor(s): Xiaodong Wu, Yanwei Huang, Zuoben Yu, Changkao Mu, Weiwei Song, Ronghua Li, Lei Liu, Yangfang Ye, Ce Shi, Chunlin WangAbstractAnti-lipopolysaccharide factors are effective antimicrobial peptides that can bind and neutralize lipopolysaccharide (LPS). In the present study, a new sequence encoding for ALF (designated as PtALF8) was cloned by suppression subtractive hybridization method using ovary of swimming crab Portunus trituberculatus as material. The full-length cDNA of PtALF8 consisted of 531 bp with an ORF of 348 bp encoding a peptide of 115 amino acids containing a putative signal peptide of 19 amino acids. The mature PtALF8 had a predicted molecular weight (MW) of 11.28 kDa and theoretica...

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