c-Jun regulates the promoter of small subunit hemocyanin gene of Litopenaeus vannamei

Publication date: January 2019Source: Fish & Shellfish Immunology, Volume 84Author(s): Peikui Yang, Defu Yao, Jude Juventus Aweya, Fan Wang, Pei Ning, Shengkang Li, Hongyu Ma, Yueling ZhangAbstractHemocyanin (HMC) is a respiratory glycoprotein, which also plays multifunctional non-specific innate immune defense functions in shrimp. However, the transcriptional regulatory mechanisms of the hemocyanin gene expression have not been reported. In the present study, we cloned a 4324 bp fragment of small subunit hemocyanin (HMCs) gene of Litopenaeus vannamei including the 5′-flanking region, from upstream 2475 bp to downstream 1849 bp (exon 1-intron 1-exon 2) by genome walking method. Four deletion constructs were then generated and their promoter activity assessed using the luciferase reporter...

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