Interleukin-4 (IL-4) may regulate alternative activation of macrophage-like cells in chickens: a sequential study using novel and specific neutralizing monoclonal antibodies against chicken IL-4

Publication date: Available online 29 October 2018Source: Veterinary Immunology and ImmunopathologyAuthor(s): Atul A. Chaudhari, Woo H. Kim, Hyun S. LillehojAbstractIn mammals, alternatively activated macrophages (AAMs) are well-recognized and are produced by stimulation with Th2 cytokines such as interleukin 4 (IL-4) and IL-13. On their mammalian counterparts, AAMs in chickens has neither been reported nor the functionality of chicken IL-4 (chIL-4) has been studied till date. Therefore, present study developed mouse monoclonal antibodies (mAbs) against chIL-4 and used these antibodies to investigate whether chIL-4 induces activation of HD11 chicken macrophage cell line. Upon characterization of mAbs using western blot, immunocytochemistry (ICC), flow cytometry and capture ELISA, activatio...

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