Leonurine inhibits IL-1 β induced inflammation in murine chondrocytes and ameliorates murine osteoarthritis.

This study aimed to evaluate the therapeutic effect of leonurine on chondrocytes and in murine OA models. Murine chondrocytes were pre-treated with leonurine (5, 10, and 20 μM) for 2 h and then stimulated with IL-1β for 24 h. Production of NO, PGE2, IL-6, TNF-α, MMP-3, MMP-13, and ADAMTS-5 was assessed with the Griess reagent and ELISAs. The mRNA expression of COX-2, iNOS, MMP-3, MMP-13, ADAMTS-5, aggrecan, and collagen-II was tested with real-time polymerase chain reaction. The protein expression of iNOS, COX-2 and NF-κB-related signaling molecules was measured with western blotting. In this study, leonurine visibly inhibited the IL-1β-induced production of NO, PGE2, IL-6 and TNF-α; and decreased the expression of iNOS, COX-2, MMP-3, MMP-13 and ADAMTS-5 in chondrocytes. Furthe...

from #Head and Neck by Sfakianakis via simeraentaxei on Inoreader https://ift.tt/2OzF9VF