Bordetella bronchiseptica Bcr4 antagonizes the negative regulatory function of BspR by its role in the type III secretion

AbstractBordetella species, includingB. pertussis, have a type III secretion system that is highly conserved among Gram ‐negative pathogenic bacteria. Genes encoding the component proteins of a type III secretion system are localized at thebsc locus in theBordetella genome. Here we investigated the function of a hypothetical protein Bcr4 encoded at thebsc locus in theB. bronchiseptica genome. We created a Bcr4 ‐deficient mutant and determined the amounts of type III secreted proteins (e.g., BopB, BopN, and Bsp22) in both the supernatant fraction and whole‐cell lysates of the Bcr4‐deficient mutant. The levels of these proteins were significantly lower than those of the wild‐type strain. In a Bcr4‐ overproducing strain, the amounts of type III secreted proteins in the supernatant...

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