A tetrameric form of CD40 ligand with potent biological activities in both mouse and human primary B cells

Publication date: January 2019Source: Molecular Immunology, Volume 105Author(s): Nannan Lai, Qing Min, Ermeng Xiong, Jun Liu, Lumin Zhang, Shoya Yasuda, Ji-Yang WangAbstractCD40 ligand (CD40 L) expressed by activated T cells interacts with CD40 on B cells and triggers B cell survival, proliferation and differentiation. Deficiency in CD40 L or CD40 in humans causes hyper IgM syndrome due to a defect in T-B interaction that is essential for Ig gene class switch recombination (CSR). CD40 L belongs to the tumor necrosis factor family and normally forms a homotrimer on the cell surface, which is important for its biological activity. To generate a multimeric CD40 L that can be used to stimulate both mouse and human B cells, we fused the extracellular domain of mouse CD40 L, which is k...

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