Association study of cell cycle proteins and human papillomavirus in laryngeal cancer in Chinese Population

Abstract

Objective

To investigate the role of human papillomavirus (HPV) in laryngeal squamous cell carcinoma (LSCC) and analyze the relationship between HPV and the expression of cell cycle‐related proteins.

Design

The study consisted of LSCC between 2005 and 2011 in Tongren Hospital. Clinical data such as age, sex, smoking/ alcohol consumption and TNM stage were collected. HPV DNA and cell cycle‐related proteins were assessed in terms of clinical features.

Setting

Single center study.

Participants

A total of 332 LSCC patients were included in the study.

Main outcome measures

The presence of genotype‐specific HPV DNA was evaluated using PCR‐RDB in formalin‐fixed paraffin‐embedded tissues. All samples were also evaluated for p16^INK4A, p21^WAF1/CIP1, P53, Cyclin D1, and Ki67 immunohistochemical staining by tissue microarray.

Results

HPV DNA was detected in 45 of 332 (13.55%) patients with LSCC, with HPV‐16 being the predominant genotype. The presence of HPV‐16 DNA was significantly associated with basaloid squamous cell carcinoma and cystic lymph node metastasis (P < 0.05). Of the 332 patients 36 (10.84%) were scored as p16^INK4A positivity, and they were more likely to be female (P < 0.05). Cyclin D1‐positivity and p21^WAF1/CIP1‐positivity were observed in 60.24% (200/332)and 40.66%(135/332), respectively. In 114 cases (34.33%), LSCCs had moderate‐ to ‐strong p53 accumulation, which was correlated with TNM stage (P < 0.05). HPV‐16 DNA was correlated with p16^INK4A, and manifested a higher Ki‐67 labeling index and p21^WAF1/CIP1 expression than HPV‐16‐negative tumors (P < 0.05). No relationship was observed between Cyclin D1or P53 expression and HPV‐16 infection (P > 0.05).

Conclusion

HPV DNA was detected in 13.55% patients with LSCC, with HPV‐16 being the predominant genotype and it was correlated with p16^INK4A, and manifested a higher Ki‐67 labeling index and p21^WAF1/CIP1 expression than HPV‐16‐negative tumors.

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