Altered Expression of miR-326 in T Cell-Derived Exosomes of Patients with Relapsing-Remitting Multiple Sclerosis

Background/Aim: Multiple sclerosis (MS) is an immune-mediated neurodegenerative disease of central nervous system (CNS). Invasion of autoreactive CD4⁺ T cells into CNS is believed to be an underlying pathogenic mechanism in MS. CD4+ T cells release exosomes which are highly enriched in microRNAs, reflective of physiological or pathological condition. Exosomal microRNAs are transferred between cells and affect the physiology of target cells. Thus exosomes could be potent agents to provide quantitative and qualitative information about immune cells involved in MS. We investigated the expression of pathogenic microRNAs in T cells-derived exosomes of MS patients or healthy controls.

Methods:  Conventional T cells (Tconv) derived from relapsing-remitting (RR) MS patients or healthy controls were cultured for 3 days by soluble anti-CD3/CD28. Exosomes were purified from supernatants. After RNA extraction from exosomes pellet, the expression levels of miR-146a, miR-29a, miR-155, and miR-326 were quantified by real-time PCR.

Result: A statistically significant increased expression of miR-326 in  Tconv- derived exosomes was observed in RRMS patients as compared with controls (7.5±1.88vs 2.51±0.9 P=0.03), On the contrary, no differences were found in the expression levels of miR-155, miR-146a, and miR-29a, in Tconv-derived exosomes of  patients as compared with controls (P>0.05). 

Conclusion: Our results point to an altered expression in exosome-derived microRNAs. MiR-326 was previously shown to play a role in the immunopathogenesis of MS by inducing TH17 differentiation and maturation. Therefore, miR-326 containing exosomes might also be a potential clinical target in course of MS. Moreover, the deregulation of this miRNA in exosomes may serve as a diagnostic and prognostic biomarker.

from #Head and Neck by Sfakianakis via simeraentaxei on Inoreader http://bit.ly/2GurtHE