Development of an in Vitro Bio-Assay Using Human Intestinal and Immune Cell-Lines to Measure the Immuno-Pathogenicity of Food Allergens

Food allergies are a rapidly growing public health problem that affect>15 million Americans. The FDA 's Food Allergen Labeling and Consumer Protection Act requires that foods containing allergenic proteins derived from the eight major food allergens be declared. The currently used Immunochemical methods (e.g. ELISA) detect IgG antigenic epitopes, and not allergenic elements. Hence, immunochemical methods may not detect antigenic epitopes that are transformed during food processing, while the immuno-pathogenicity could continue to persist. (Source: Journal of Allergy and Clinical Immunology)

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